Growth results of SP2/0-Ag14 cells
01. Test methods
1. Medium Preparation of 10% serum +DMEM high-sugar medium
2. Cell passage method ratio 1:8 passage; Pass once in 72h.
Take a bottle of cells, discard the culture supernatant, and add 2ml DPBS to wash twice. Add 1ml pancreatic enzyme digestion cells, and when the cells fall out of the culture bottle, add 1ml bovine serum to terminate digestion. Add 9ml DPBS to blow the culture bottle wall to make cell suspension, and transfer the cell suspension into a 15m centrifuge tube and centrifuge at 1000rpm for 5 minutes. The supernutriment was absorbed, 10ml of suspension cells were added to the medium, 0.1ml of cell suspension was absorbed and mixed with an equal volume of Trypan blue for counting. Adherent cells Suspension cells.
A bottle of cells was taken, and then added to the centrifuge tube, centrifuged at 1000 RPM for 5 minutes, then the supernatant was absorbed, 10ml of the suspended cells were added to the medium, and 0.1ml of the cell suspension was absorbed and mixed with the same volume of Trypan blue for counting.
60mm cell culture dish with 5ml medium.
Culture system cultivation conditions
37 ° C, 5% CO2.
02. Test results
03. Cell state
Recommended product
Globekang Fetal Bovine Serum F800820
(1) Standard imported high-end fetal bovine serum.
(2) Widely used in a variety of cell cultures, including some stem cells, primary cells and other delicate cells.
(3) Blood collection of fetal cattle at 6-8 months of gestational age.
(4) C+A grade purification workshop automatic filling line production, batch 4000 bottles, the difference between batches is very small.
(5) After 0.1micron 3 times sterilization filtration, comprehensive physical and chemical, microbial testing.
(6) Very low endotoxin, no mycoplasma, no specific antibody.
To obtain the COA of F800820 product, please contact customer service!
