Vero Cell Growth Performance Data

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Part.01

Experimental method

Medium configuration

10% serum +MEM medium

Cell passage method

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scale

1:4 generations

Pass once in 72h

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Adherent cell

Take a bottle of cells, discard the culture supernatant, and add 2ml DPBS to wash twice. Add 1ml pancreatic enzyme digestion cells, and when the cells fall out of the culture bottle, add 1ml bovine serum to terminate digestion. Add 9ml DPBS to blow the culture bottle wall to make cell suspension, and transfer the cell suspension into a 15m centrifuge tube and centrifuge at 1000rpm for 5 minutes. The supernutriment was absorbed, 10ml of suspension cells were added to the medium, 0.1ml of cell suspension was absorbed and mixed with an equal volume of Trypan blue for counting.

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Suspension cell

A bottle of cells was taken, and then added to the centrifuge tube, centrifuged at 1000 RPM for 5 minutes, then the supernatant was absorbed, 10ml of the suspended cells were added to the medium, and 0.1ml of the cell suspension was absorbed and mixed with the same volume of Trypan blue for counting.

Cultivation system

60mm cell culture dish with 5ml medium.

Culture condition

60mm cell culture dish with 5ml medium.

Part.02

Experimental Result